产品中心
产品查询/报价
您的姓名
学校/机构及部门名称
联系方式
查询产品
留言内容
提交

Aurora (小鼠)整体动物肌肉测试系统 (1300A )

能够弹性地应用“活体,原位,离体”三种不同的实验技术去准确地测量动物的肌肉性能

0.00
0.00
  
产品简介

能够弹性地应用“活体,原位,离体”三种不同的实验技术去准确地测量动物的肌肉性能



一个多功能性的系统: 能够性地用 “活体,原位,离体”三种不同的实去准确地齿类动物的肌肉性能


1300A 1305A 是高性能和高精确度的测试系统,研究者利用要的方法去量肌肉的力和各种力学性能

通过把活体肌肉测试,原位肌肉测试和离体肌肉测试三种技术融合到一个系统里面,使研究者能够捕捉到啮齿类动物肌肉生理的全貌。测试系统配备针对小鼠或大鼠的实验平台装置(可与控温器组合),而且能配合不同的夹器把动物的身体和後肢固定在一个平台上,得以在实验当中保持稳定。另外,透过简单的置换,可以把装置的动物平台更换成
一个
25ml容积的横向浴盆,去进行离体肌肉测试实验。这个系统更应用了Aurora Scientific 的旗舰双模肌肉杠杆系统; 一个双模传感器,不仅能测量力度和长度,同时也有控制力度和长度的能力。


建立於双模感器强大能力的基上,肌肉样本和双模感器之间只需要一个接点,就能够同行力度和长度的量和控制,大幅减省了实操作程,同也提高了据生率。除此之外,系统也备有高能双相刺激器和相的电极。


数据采集方面,控制和分析软件会预先安装在电脑主机之中。实验前操作准备,数据采集和数据分析都能通过我们的控制和分析软件(DMC/DMA)在数分钟内完成。静息长度,静息力度,刺激等参数和进行实验的方案都可以通过控制软件(DMC)去设置。电脑桌面也会提供标准实验方案的扩充程式库,方案包括: 抽搐张力,强直收缩张力,疲劳,力-刺激频率,力-收缩速率,硬度和功循环等。
 



* 强大的三合一融合设间和空间的同提高了生

* 高实
验产

* 高速的
据采集和分析件: 用於Windows Linux 操作系统

* 双用刺激器
: 或神经行刺激

* 可
行理想的活体动物实方案: 精密且可控温的平台

* 在一个系统的基
上,可以测试小鼠和大鼠的配置

* 测量的力值峰度: 0.5N10N


能够进行更多复杂且多方面测量的方案

* 测量力度峰值可达10N(1000g)


3-in-1 in-situ System Enables Biologist to Test Physiology of Muscle Regeneration

CHALLENGE

As a molecular and developmental biologist, Dr. Chen-Ming Fan of the Carnegie Institution for Science studies the molecular mechanisms involved in mammalian development, most notably in the musculoskeletal system. Over the years Dr. Fan has uncovered developmental mechanisms that are involved in muscle regeneration through the skeletal muscle stem cell niche, as regeneration is thought to be a recapitulation of development processes.

After identifying mechanisms that may aid in skeletal muscle regeneration, Dr. Fan needed a way to test if muscle function was improved or restored in aged and diseased mouse models.

SOLUTION

With the ability to test muscle function in-situ and in-vivo, the 1300A Whole Animal System was the logical choice for Dr. Fan to expand his research from molecular mechanisms to functional measurements. The in-situ setup would allow Dr. Fan to measure force produced by specific muscles in the hindlimb while keeping the muscle’s blood supply and nervous input intact. This represents a more physiological environment than the standard isolated muscle experiments. In addition, measurements of aggregate hindlimb function without surgery using the in-vivo setup would permit longitudinal study of muscle regeneration and functional recovery in aged and disease states.

RESULTS

Once the whole animal system was installed and demonstration complete, Dr. Fan and his students were extremely excited about the prospect of the system and began making functional measurements immediately. The lab employed the in-situ technique to measure functional recovery of the Tibialis Anterior (TA) following experimentally induced muscle injury in aged and dystrophic mice. Just over a year later, Dr. Fan’s lab published a paper in Nature Medicine citing our 1300A system. Dr. Fan stated it would not have been possible to publish this article without the 1300A whole animal system and he continues to utilize this powerful system to further test the mechanisms involved in muscle regeneration.



* Rooney, Jachinta and Rich Lovering. “Single muscle contractile measurements in vivo and in situ.” NIH SOP: MDC1A_M.2.2.002 (2015): 1-8.
Al-Sajee, Dhuha et al. “Xin-deficient mice display myopathy, impaired contractility, attenuated muscle repair and altered satellite cell functionality.” Acta Physiologica 214.2 (2015): 248-260.
Thomas, Melissa M. et al. “Early oxidative shifts in mouse skeletal muscle morphology with high?fat diet consumption do not lead to functional improvements.” Physiological Reports 2.9 (2014): e12149.
Ambrosio, Fabrisia et al. “Arsenic induces sustained impairment of skeletal muscle and muscle progenitor cell ultrastructure and bioenergetics.” Free Radical Biology and Medicine 74 (2014): 64-73.
Distefano, Giovanna et al. “Neuromuscular Electrical Stimulation as a Method to Maximize the Beneficial Effects of Muscle Stem Cells Transplanted into Dystrophic Skeletal Muscle.” PLoS ONE 8.3 (2013): e54922.
Alway, Stephen E. and Robert G. Cutlip. “Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle.” Methods in Molecular Biology Myogenesis (2011): 185-211.




          

下一个:
客服中心

Hotline / 热线电话

中国: 020-87357737

9:00 AM - 18:00 PM


香港: 852-27239888

9:30 AM - 18:30 PM